| First Author | Fukushima T | Year | 2019 |
| Journal | Cell Rep | Volume | 29 |
| Issue | 12 | Pages | 4144-4158.e7 |
| PubMed ID | 31851939 | Mgi Jnum | J:300785 |
| Mgi Id | MGI:6488927 | Doi | 10.1016/j.celrep.2019.11.061 |
| Citation | Fukushima T, et al. (2019) Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep 29(12):4144-4158.e7 |
| abstractText | Quiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between "dormant" and "active" HSCs remains unresolved. Here we generate a G0 marker (G0M) mouse line that visualizes quiescent cells and identify a small population of active HSCs (G0M(low)), which are distinct from dormant HSCs (G0M(high)), within the conventional quiescent HSC fraction. Single-cell RNA-seq analyses show that the gene expression profiles of these populations are nearly identical but differ in their Cdk4/6 activity. Furthermore, high-throughput small-molecule screening reveals that high concentrations of cytoplasmic calcium ([Ca(2+)]c) are linked to dormancy of HSCs. These findings indicate that G0M separates dormant and active adult HSCs, which are regulated by Cdk4/6 and [Ca(2+)]c. This G0M mouse line represents a useful resource for investigating physiologically important stem cell subpopulations. |
