| Primary Identifier | MGI:6711969 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | STOCK Gt(ROSA)26Sor<tm12Luo>/Luo |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | This allele was produced in zygotes collected from mice carrying the Gt(ROSA)26Sortm12Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl+)-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, and DBA/2J. |
| molecularNote | The allele was created to have the human BEST1 promoter/enhancer regions directing the expression of a CreER(T2) fusion gene. The targeting vector contained, from 5' to 3', the human BEST1 promoter (nucleotides -585 to +38), followed by a CreER(T2) fusion gene, and an SV40 a polyA cassette. The plasmid was pronuclear injected into C57BL/6J(attPx3) zygotes along with phiC31 integrase mRNA. The resulting founder mice were bred with C57BL/6J for germline transmission. |
