| Primary Identifier | MGI:6763413 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | STOCK Gt(ROSA)26Sor<tm12Luo>/Luo |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | This allele was produced in zygotes collected from mice carrying the Gt(ROSA)26Sortm12Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl+)-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, and DBA/2J. |
| molecularNote | Using the TARGATT integrase-mediated cassette exchange, the targeting construct containing a bidirectional tetO (TRE) promoter directing the expression of human SHH cDNA and a ZsGreen1 fluorescent marker gene cassette was inserted into Gt(ROSA)26Sor docking attP site using phiC31 integrase. At least three founder lines were generated with line2 and 3 showing similar reporter expressions. Line 2 was used for most of the study. |
