| Primary Identifier | MGI:7516071 | Allele Type | Endonuclease-mediated |
| Attribute String | Recombinase, Reporter | Gene | Fcgr1 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting construct inserted IRES, tdTomato, T2A self-cleaving peptide, iCre recombinase, and an FRT site-flanked PGK-NEO-BGHpA cassette downstream of the stop codon of the Fcgr1 gene by CRISPR������Cas9-mediated homologous recombination in mouse ES cells. Mice harboring the targeted allele were subsequently crossed with Rosa26FLPe mice to remove the neo cassette. However, analysis of the mice revealed that a neo sequence closely linked to (or elsewhere within) the Fcgr1-cre allele is present. It has not been determined if the retained neo is a full or partial sequence, nor is it known where it lies near/within the Fcgr1-cre allele. |
