| Primary Identifier | MGI:7865855 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Tmc1-Tmc2/Tmc1*)1Ajg |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | BAC clone MSMg01-526L10 (181,748 bp, Riken Bioresource Center DNA Bank, Tsukuba, Japan), encoding wild-type MSM/Ms mouse genomic Tmc1 (170,745 bp) was used to construct Tg[Tmc1deltaEx8_9], Tg[PTmc1::Tmc2] using the Counter-Selection BAC Modification Kit with Red/ET recombination technology (Gene Bridges, Heidelberg, Germany). To construct Tg[Tmc1deltaEx8_9], 2775 bp including exons 8 and 9 were deleted using the same method. To construct Tg[PTmc1::Tmc2], Tg[Tmc1deltaEx8_9] was modified to replace the translation initiation codon of Tmc1 with the Tmc2 cDNA (NM_138655.1) conjugated with the SV40 polyadenylation signal, respectively. |
