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Allele : Gt(ROSA)26Sor<em22Arz> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 22, Aamir Zuberi
Primary Identifier  MGI:8262940 Allele Type  Endonuclease-mediated
Attribute String  RMCE-ready Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6NJ Is Recombinase  false
Is Wild Type  false
molecularNote  Using CRISPR/Cas9 endonuclease-mediated genome editing/oligo-mediated homology directed repair. First, sgRNAs (ACTCCAGTCTTTCTAGAAGA and CTCCAGTCTTTCTAGAAGAT) were selected to target intron 1 of the Gt(ROSA)26Sor locus on Chromosome 6 and a long single stranded (lss) DNA donor was used to integrate the Bxb1 attB-GT site into the resulting dsDNA break.
  • mutations:
  • Insertion
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

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