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Allele : Gt(ROSA)26Sor<em2(CAG-Htr4*/GFP,-jRGECO1a)Yuli> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Yulong Li
Primary Identifier  MGI:8246660 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6NCrl Is Recombinase  false
Is Wild Type  false
molecularNote  Using CRISPR/Cas9 methodologies (sgRNA: AAGGCCGCACCCTTCTCCGG AGG), the targeting construct composed of (from 5' to 3') CAG-loxP-STOP-loxP-gGRAB)5-HT3.0(-iP2A- jRGECO1a-WPRE-bGH pA was inserted into Gt(ROSA)26Sor locus. The STOP codon is composed of 5xSTOP sequences. jRGECO1a encodes a red fluorescent calcium sensor. gGRAB)5-HT3.0( encodes a modified metabotropic 5-HT receptor fused to circularly permuted green fluorescent protein (cpEGFP).
  • mutations:
  • Insertion
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1 Feature

Genome

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0 Mutation Involves

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