| Primary Identifier | MGI:8252910 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| description | Embryonic stem cells = A-A02? made for us by Taconic GmbH |
| molecularNote | The allele consists of the CAG, floxed STOP-MetRS(L274G)-T2A-MetRS(L274G)-P2A-MetRS(L274G)-E2A-EGFP (WPRE ) inserted into the mouse ROSA26 locus using recombination-mediated cassette exchange in embryonic stem cells. The vector is designed to contain a mutant F3 FRT site, a NEO selection cassette, CAG promoter, a floxed STOP cassette, triple repeat of the mouse methionine-tRNA synthetase (Mars1) with the L274G point mutation separated by T2A, P2A, followed by E2A peptide sequence (to mediate ribosomal skipping) followed by Green Fluorescent Protein coding sequence, WPRE and an FRT site. This vector was integrated into the Gt(ROSA)26Sor locus by recombinase mediated cassette exchange (RMCE). The L274G mutation allows the binding of the methionine surrogate azidonorleucine (ANL) onto the methionine tRNA. ANL can be tagged for imaging or affinity purification. |
