| Primary Identifier | MGI:8290424 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Plxnd1 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting vector contains, from 5' to 3', a partial Plxnd1 sequence spanning exon 1 through part of exon 2 up to (but not including) the endogenous stop codon,a T2A sequence, a flpO recombinase gene (a codon-optimized FLPe gene), a BHG polyA sequence, an AttB site, a PGK-neomycin cassette, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL). |
