| Primary Identifier | MGI:8293347 | Allele Type | Endonuclease-mediated |
| Attribute String | Not Specified | Gene | Dmxl1 |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR-targeting introduced the mutation (TTT to TCT) responsible for the amino acid substitution of phenylalanine with cysteine at position 1729 (F1729C). Quantitative real-time PCR analysis of craniofacial tissues at E13.5 shows a ~25% reduction of Dmxl1 expression in heterozygous embryos, and a mild (~22%) increase of Dmxl1 expression in homozygous embryos. |
