| Primary Identifier | MGI:8293348 | Allele Type | Endonuclease-mediated |
| Attribute String | Hypomorph | Gene | Dmxl1 |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR-targeting introduced the mutation (GCA to ACA) responsible for the amino acid substitution of alanine with threonine at position 2330 (A2330T). Quantitative real-time PCR analysis of craniofacial tissues at E13.5 shows a ~25% and ~40% reduction of Dmxl1 expression in heterozygous and homozygous embryos, respectively, suggesting that this could be a hypomorphic allele with sufficient transcript levels and/or protein activity for DMXL1 function and animal survival. |
