| First Author | Stewart L | Year | 2005 |
| Journal | J Neurochem | Volume | 94 |
| Issue | 6 | Pages | 1739-45 |
| PubMed ID | 16026391 | Mgi Jnum | J:101317 |
| Mgi Id | MGI:3603740 | Doi | 10.1111/j.1471-4159.2005.03336.x |
| Citation | Stewart L, et al. (2005) Runx1 expression defines a subpopulation of displaced amacrine cells in the developing mouse retina. J Neurochem 94(6):1739-45 |
| abstractText | AML1/Runx1 (Runx1) is a mammalian transcription factor that plays critical roles in regulating the differentiation of a number of different cell types. In the present study, we have utilized mice expressing beta-galactosidase (beta-gal) under the control of the Runx1 promoter to characterize the spatiotemporal expression pattern of Runx1 during retinogenesis. Expression of beta-gal was first detected at embryonic day 13.5 in post-mitotic cells located in the inner retina and overlapped with expression of the early amacrine and ganglion cell marker protein Islet1. During subsequent developmental stages, the number of beta-gal-positive cells increased in a central-to-peripheral gradient until late embryogenesis but then decreased in the early post-natal retina. beta-gal-positive cells were located primarily in the ganglion cell layer by late embryonic/early post-natal stages and were identified as a subpopulation of displaced amacrine cells by the continued expression of Islet1, as well as Pax6, and the coexpression of the amacrine cell subtype-specific markers choline acetyltransferase, calretinin and the 65-kDa isoform of glutamic acid decarboxylase. These findings identify Runx1 as a novel marker for a restricted amacrine cell subtype and suggest a role for this gene in regulating the post-mitotic development of these cells. |
