Primary Identifier | MGI:2180099 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Adh1 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
description | A modified R1 ES cell line dervived from (129X1/SvJ x 129S1/Sv)F1-Kitl+ containing a targeted Adh3 (class III) locus was used. |
molecularNote | Exons 7 through 9 and the polyadenylation signal were replaced with a PGK-hygro gene downstream of exon 6. This construct was developed by altering a previously described construct, tm1Due, containing a neomycin selection gene, for use in the generation of a double knockout. |