Primary Identifier | MGI:2149755 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Jun |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed PGK-betageo selection cassette was inserted into position -360 of the 5' UTR of the gene. In addition, serine codons 63 and 73 were changed to alanines (S63A and S73A) by site directed PCR-based mutagenesis. In this allele the floxed PGK-betageo cassette was removed by pronuclear injection of a cre-expressing plasmid. |