| Primary Identifier | MGI:2158738 | Allele Type | Targeted |
| Attribute String | Dominant negative | Gene | Ikzf1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An 8.5 kb genomic fragment containing part of exon 3 and exon 4 was replaced with a neomycin cassette. The mutation deletes zinc fingers 1, 2, and 3, which mediate the high affinity binding domain of the protein. Mutant mRNA was detected by RT-PCR analysis. Low amounts of mutant protein were detected by western analysis. Gel retardation assays using nuclear extracts from spleen and thymus showed no binding activity in homozygous mutant animals. A subsequent study suggested this allele generates a dominant-negative protein. |
