Primary Identifier | MGI:2179218 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rad51 |
Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | 54 nucleotides of exon 5 and 187 nucleotides of intron 5 were replaced with a neo-derived cassette via homologous recombination resulting in the deletion of the ATP-binding motif of domain II responsible for DNA repair and recombination. Homozygous mutant embryos could only be detected before the 4- to 8- cell stage by PCR analysis. |