| Primary Identifier | MGI:2180116 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tle5 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin selection cassette replaced exon 2 and also introduces both termination and polyadenylation signals into the open reading frame. The authors noted that if the aforementioned signals were not recognized, the splicing of exons 1 and 3 would result in frameshift mutation. Western analysis of lysates isolated from the brains and spleens of homozygous mutant mice showed an absence of protein expression. |
