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Allele : Nefm<tm1Ral> neurofilament, medium polypeptide; targeted mutation 1, Robert A Lazzarini

Primary Identifier  MGI:2180135 Allele Type  Targeted
Attribute String  Null/knockout Gene  Nefm
Transmission  Germline Strain of Origin  (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Is Recombinase  false Is Wild Type  false
molecularNote  Exon 1 was disrupted by insertion of a PGK-neo cassette by homologous recombination. RNase protection assays using probes to exon 1 and exon 3 did not detect gene expression in brain of homozygous mutant animals. Western blot analysis and immunohistochemistry of brain sections from homozygous mutant animals confirmed the absence of protein expression.
  • mutations:
  • Insertion
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

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7 Publication categories