Primary Identifier | MGI:2180501 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Il5ra |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A stop codon was introduced into exon 5 and a neo cassette was inserted into exon 6 via homologous recombination. The neo insertion disrupts 4 cysteine residues that are conserved within the cytokine receptor family. Absence of gene expression was verified by flow cytometry analysis of resting peritoneal cells from homozygous mutant animals. |