| Primary Identifier | MGI:2181680 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Usf2 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | 11 kb of the gene, including most of the 5' untranslated region and all of the coding exons, was deleted and replaced with a PGK-neo cassette via homologous recombination. The absence of gene expression in homozygous mutants was confirmed by electrophoretic mobility shift assay (EMSA) and Western blot analysis of nuclear extracts from mutant mouse embryonic fibroblasts (MEF). |
