| Primary Identifier | MGI:2182548 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Itgb8 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = JMA7. |
| molecularNote | A PGK-neo-cassette replaced sequences encoding 45 amino acids at the C-terminus, corresponding to exon 4, which includes a von Willebreand factor-A-like domain, and part of the intron sequences. This targeting event resulted in a frameshift in addition to a deletion, abolishing functional Itgb8 protein. Northern blot and RT-PCR analysis verified the lack of functional transcript in homozygous mice. |
