| Primary Identifier | MGI:2182488 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Piwil1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | 15.5 kb of the gene spanning exons 3-22 were replaced with a loxP-flanked PGK-neo cassette preceded by a GFP reporter gene. The targeting event results in a GFP fusion product after the first 9 amino acids of the gene. Western blot and immunofluorescent microscopy of testis from homozygous mutant animals verified the absence of Piwil1 expression. |
