| Primary Identifier | MGI:2386175 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Upf1 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 4 through 6 were replaced with a neomycin selection cassette. Northern blot analysis of MEF cell lines derived from heterozygous mutant mice showed that aberrant transcripts were produced from the targeted allele. RT-PCR and sequencing analysis of the these transcripts identified chimeric transcripts that contained the neo gene as well as splice variants in which exon 3 joined exon 8. In either case, a premature stop codon was introduced immediately downstream of exon 3. |
