| Primary Identifier | MGI:2388394 | Allele Type | Targeted |
| Attribute String | Conditional ready, Modified regulatory region, No functional change | Gene | Igh |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | The targeted IgH allele was of the a allotype. |
| molecularNote | The endogenous locus was modified via homologous recombination and subsequent in vitro cre-mediated excision to generate a floxed version of Ightm2Cgn. The region flanked by single loxP sites contained the inserted variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8) as well as the Igh intron enhancer (Emu or Rr150). Flow cytometry verified cell surface expression on peripheral B cells obtained from heterozygous and homozygous mice. |
