Primary Identifier | MGI:2445941 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Arid5b |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 98 bp exon within the AT-rich interaction domain (ARID) was replaced with a neomycin selection cassette inserted by homologous recombination. RT-PCR of RNA obtained from primary embryonic fibroblasts confirmed the removal of the targeted exon. Sequence analysis identified a resultant frameshift mutation. |