| Primary Identifier | MGI:2447175 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Snai2 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 2 kb region of the gene, which includes part of exon 2 and exon 3 and corresponds to the zinc finger coding region, was replaced with an in-frame lacZ sequence and PGK-neo cassette. The resulting targeted allele is expected to produce a fusion protein composed of the first 120 amino acids from Snai2 and beta-galactosidase. Whole mount in situ hybridization analysis of mutant embryos showed that the lacZ expression pattern followed that of endogenous Snai2. |
