| Primary Identifier | MGI:2447787 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Null/knockout | Gene | Ikzf1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The floxed neo cassette in exon 2 was removed by mating Znfn1a1tm1(Pax)Mbu animals to cre expressing line TgN(Sycp1-cre)4Min, thus allowing expression from the Pax5-GFP cassette inserted at Znfn1a1. Western blot analysis detected Pax5 protein expression in thymocytes and myeloid cells at levels similar to wild-type. GFP expression in B cells was detected by flow cytometric analysis. |
