| Primary Identifier | MGI:2653959 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Rr27 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | Disruption of the Ctcf target sites within the imprinting control region results in a loss of Ifg2 and H19 imprinting when the mutant allele is passaged through the female germline. The loss of imprinting is associated with an 11% increase in neonatal body weight. |
| molecularNote | The first, third, and fourth Ctcf target sites within the imprinting control region (ICR) were modified from GTGG to ATAT via site directed mutagenesis. A floxed neomycin resistance gene cassette that was included in the targeting vector for selection was excised via in vivo cre-mediated recombination in the germline. |
