| Primary Identifier | MGI:2655218 | Allele Type | Targeted |
| Attribute String | Conditional ready, Modified regulatory region, No functional change | Gene | Rr27 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The differentially methylated region (DMR), located upstream of the H19 transcription initiation site, was left flanked by single loxP sites at -6.5 to -0.8 kb upstream of H19 major start within the regulatory region 27 (Rr27) and a neomycin-resistance gene flanked by loxP sites inserted at the -7.0kb upstream of H19. The neo-cassette was removed in ES cells by transiently transfecting with a Cre recombinase-expressing plasmid. |
