Primary Identifier | MGI:2660773 | Allele Type | Targeted |
Gene | Jun | Transmission | Germline |
Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The entire gene coding region was deleted and replaced with a Junb floxed PGK-neo knock-in cassette via homologous recombination. The floxed neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Northern blot analysis confirmed the expression pattern of the Junb knock-in sequence follows that of the endogenous gene. |