| Primary Identifier | MGI:2682898 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hmmr |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A genomic fragment encompassing exons 8 through 16 was replaced with a Hprt minigene. Full length transcript was undetected by RT-PCR analysis of homozygous mutant spleen tissue. Analysis of homozygous mutant MEFs identified aberrant transcript in which exon 7 had spliced to exon 17. Sequence analysis indicated a frameshift mutation at the aberrant splice junction, affecting downstream sequence encoding the HA-binding region. Western blot analyses of homozygous spleen tissue and MEFs did not detect protein corresponding with the mutant transcript. |
