| Primary Identifier | MGI:2683633 | Allele Type | Targeted |
| Attribute String | Modified regulatory region | Gene | Rr27 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | This locus is subject to imprinting, however the introduced mutations at the AB sites did not affect the establishment or maintenance of the male-specific epigenetic state in the sperm. |
| molecularNote | Mutations were introduced at each of the 3 AB sites (AB-1, AB-2, and AB-3) located in the imprinting control region (ICR) upstream of the H19 transcription start site. Single loxP sites remained flanking the ICR after a floxed cassette containing a neomycin resistance gene and a cre recombinase gene was self-excised. The mutations were shown by EMSA to ablate binding activity. Imprint establishment and maintenance was unaffected in mutant mice. |
