| Primary Identifier | MGI:2684421 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rac1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Targeting took place in ES cells carrying the cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase only in haploid spermatids. A loxP site was introduced between exons 3 and 4 while a floxed PGK-neomycin resistance gene was introduced in reverse orientation between exons 5 and 6. Cre recombination in the male germline removed the neomycin gene as well as exons 4 and 5. |
