| Primary Identifier | MGI:3044978 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Map3k4 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was derived from Map3k4 |
| molecularNote | A loxP site was inserted 5' to exon 3 and a floxed neomycin resistance cassette was placed 3' to the exon. Chimeric male mice were bred to female mice carrying Tg(Tek-cre)12Flv to delete exon 3 and the neomycin resistance gene. This removes the entire GADD45-binding domain and causes a frame shift and premature termination. Absence of protein was confirmed by Western blot analysis. |
