Primary Identifier | MGI:3045408 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Btla |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 2, which encodes the Ig-like domain, was disrupted by the insertion of a neo in reverse transcriptional orientation via homologous recombination. Southern blots confirmed gene targeting and RT-PCR indicated failure of mutant spleen cells to express full-length mRNA. |