Primary Identifier | MGI:3045724 | Allele Type | Targeted |
Attribute String | Modified regulatory region | Gene | Rr27 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | CTCF target site 4 within the imprinting control region (ICR) was modified from a central GTGG motif to ATAT via homologous recombination. A floxed neomycin resistance gene cassette that was included in the targeting vector was excised via in vivo cre-mediated recombination in the germ line. Southern blot analysis and sequence analysis confirmed recombination. |