Primary Identifier | MGI:3047784 | Allele Type | Targeted |
Gene | Xist | Transmission | Germline |
Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
Is Wild Type | false |
molecularNote | A sequence between 1 and 9 kb upstream of the P1 start site was deleted and replaced with a floxed PGK-neomycin resistance gene. Southern blot and PCR confirmed recombination. RT-PCR of mutants indicated antisense expression was not affected and ectopic sense transcription was detected upstream of Xist, as well as elevated transcription within the Xist locus. |