| Primary Identifier | MGI:3046937 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Grem1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | This mutation was determined to be allelic to both Fmn1ld-Is(17;In2)1Gso and Fmn1tm1.1Zlr through complementation analyses (J:91385). Transcription was not detected in the limb bud mesenchyme of either type of compound heterozygous mutant embryo. This allele is allelic to Grem1ld (J:91385) as well. In each case, compound heterozygous mutant mice display the mutant "limb deformity" limb phenotype. |
| molecularNote | The entire 552 bp CDS encoded by exon 2 and 132 bp of the 3' UTR were deleted and replaced by an IRES-lacZ cassette and a loxP site flanked neomycin resistance gene cassette. In situ hybridization of heterozygous embryos show identical distribution of wild-type and mutant allele expression. |
