Primary Identifier | MGI:3047536 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Cd79a |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | LoxP sites were inserted upstream of exons 2 and 4, surrounding the extracellular membrane and transmemebrane encoding sequences. Transient cre expression excised a floxed neo upstream of exon 2. Southern blot confirmed recombination and excision of the neo. Flow cytometry of bone marrow, blood, peritoneal and splenic B cells in homozygous mice indicated gene expression levels were normal. |