| Primary Identifier | MGI:3047537 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd79a |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Crossing Cd79atm4Cgn mice with deleter mice expressing cre in the germline created this allele in which floxed exons 2 and 3 were excised, thus removing the extracellular membrane and transmembrane encoding sequences. FACS analysis of mutant bone marrow and spleen indicated complete lack of B cell development and T cell numbers decreased to 30-50% of wild-type controls. Mu chain was detected in the cytoplasm of pro-B cells, demonstrating VDJ recombination was functional. |
