Primary Identifier | MGI:3579185 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Plxna4 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A pgk-neomycin cassette with a 5' endoplasmic reticulum retention signal and a 3' myc epitope replaced exons 18-19, which encode the transmembrane domain. RT-PCR of mutants failed to detect transcript from 3' of the insertion in mutants. Northern blot failed to detect the truncated transcript from mutants. |