Primary Identifier | MGI:3583876 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Gna13 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 4 and much of intron 3 were replaced via homologous recombination with a construct composed of an frt flanked neomycin resistance cassette, a floxed exon 4, and a segment of intron 4 equivalent to the region of the endogenous intron being replaced. Mice carrying this targeted allele were crossed to transgenic mice carrying Flp recombinase under the control of a beta-actin promoter. This results in excision of the neomycin cassette and leaves a floxed exon 4. |