| Primary Identifier | MGI:3612485 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Lrp5 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to replace the 3' part of exon 7 and 5' part of exon 8 with a lac-Z-neo cassette by homologous recombination. The vector was intended to create a Lrp5-lacZ fusion protein, however, no Lrp5-containing transcripts were detected by Northern blot, and no Lrp5 or Lrp5-lacZ fusion proteins were detected by Western blot or histochemical staining of liver and bone. |
