Primary Identifier | MGI:3662669 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mrtfa |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A vector was designed to delete a part of exon 9 and all of exon 10, which encode the basic, glutamine-rich and SAP domains. A lacZ was fused in frame with exon 9 and a neo cassette. RT-PCR demonstrated that the correct exons were deleted and that exon 8 was spliced to exon 12, thereby deleting the lacZ-neo cassette. The resulting truncated protein would be nonfunctional. |