Primary Identifier | MGI:3664444 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tnip2 |
Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
Is Recombinase | false | Is Wild Type | false |
description | PC3 ES cells are homozygous for Tg(Prm-cre)70Og, a transgene that expresses Cre recombinase only in haploid spermatids. |
molecularNote | Insertion of a targeting vector into PC3 ES cells placed a loxP site in intron 2 and a floxed neo resistance cassette in intron 5. Cre-mediated excision removed exons 3-5 upon transmission from male chimeras. Immunoblot confirmed absence of protein in mutant mice. |