| Primary Identifier | MGI:3697095 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(CAG-Bgeo,-DsRed*MST)1Nagy |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | The transgenic ES cell clone 11/C1 was used to generate transgenic mice. The authors originally identified the red fluorescent protein variant employed in constructing the transgene vector as DsRed.T3; in a later erratum, they corrected this to DsRed-MST, which differs from the former at two amino acid positions (K Vintersten et al. 2005. Genesis 42(3):218).
see MMRRC Nagy ES cell lines: ES cell line G4 with DsRed.MST transgene (http://www.mmrrc.org/strains/11988/011988.html) and ES cell line G4 with Z/red transgene (http://www.mmrrc.org/strains/11987/011987.html) - dlb |
| molecularNote | The transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed cre transgene results in fluorescence of ES cells, embryos and adult mice. |
