| Primary Identifier | MGI:3763386 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Mir155 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NTac)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A modified bacterial artificial chromosome (BAC) targeting vector was used to replace a 0.97 kb portion of exon 2 of the bic/mir-155 gene with an in-frame b-galactosidase (lacZ) reporter gene (with polyA sequence and followed by a loxP-flanked neomycin resistance cassette). The mice were bred with a cre-deleter strain (congenic C57BL/6 background) to remove the loxP-flanked selection cassette. The resulting bic/mir-155 mutant mice (with lacZ followed by a single remaining loxP site). In heterozygotes, approximately 60% of germinal center (GC) B cells express the lacZ reporter whereas the vast majority of the non-GC B cells do not. |
