| Primary Identifier | MGI:3784965 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rspo1 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was made using sequential targeting events. The first targeting event resulted in the insertion of a floxed neo cassette into exon 2 and the second event resulted in the replacement of exons 5 through 6 with a floxed puromycin resistance cassette. Transient cre expression was used to remove the selection cassettes and the remaining coding region of exon 2 and exon 3. The final allele lacks the entire coding region. The absence of transcript was confirmed by RT-PCR on ovary and testis extracts. |
