Primary Identifier | MGI:3794733 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Ppp4c |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted in between exons 4 and 5. A floxed neomycin resistance cassette was inserted downstream of exon 8 and also replaced the codons of the last 4 amino acids of Tbx6. Northern blot analysis confirmed reduced Ppp4c transcript expression in embryo. RT-PCR confirmed normal Tbx6 transcript expression. |