Primary Identifier | MGI:3808572 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | G0s2 |
Transmission | Germline | Strain of Origin | C57BL/6NTac |
Is Recombinase | false | Is Wild Type | false |
Project Collection | KOMP-Regeneron |
molecularNote | The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 329 bp between positions 192954751-192955079 of Chromosome 1 (Build GRCm39). The G0S2 coding region was replaced by a lacZ and loxP-flanked neomycin resistance gene cassette and the lacZ gene was fused in-frame at the G0S2 ATG start codon. Immunoblot analysis confirmed the absence of protein expression in gonadal fat tissue from homozygous mutant mice. |